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KMID : 0861020180330030025
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Korea Journal of Herbology
2018 Volume.33 No. 3 p.25 ~ p.35
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Molecular authentication of Lepidii seu Descurainiae Semen by the development of matK amplification primers and analysis of sequences
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Moon Byeong-Cheol
Kim Wook-Jin
Yang Sun-Gyu
Park In-Kyu
Yeo Sang-Min
Noh Pu-Reum
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Abstract
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Objectives : Lepidii seu Descurainiae Semen has been frequently adulterated with the seeds of several inauthentic plant species. However, the accurate identification of these plant seeds is very difficult. To develop a reliable genetic authentication tool for Lepidii seu Descurainiae Semen, we analyzed mat K sequence.
Methods : To obtain the mat K sequences of plant materials, genomic DNA was extracted from 24 samples and PCR amplification was carried out using matK-AF/matK-8R universal primer set and matK-LDSF/matK-LDSR primer set. For identifying species-specific nucleotides and phylogenetic analysis, matK regions were sequenced and comparatively analyzed by the ClustalW and Maximum Likelihood method.
Results : We developed a new primer set to amplify matK region in Lepidii seu Descurainiae Semen and closely related plant samples. From the comparative analysis of mat K sequences, we identified species-specific marker nucleotides for D. sophia, L. apetalum, L. latifolium, E. cheiranthoides, E. macilentum, and D. nemorosa, respectively. Furthermore, phylogenetic analysis revealed clear classification depending on the species. These results indicated that the matK sequence obtained a new primer set in this study was useful to identify Lepidii seu Descurainiae Semen in species level.
Conclusions : We developed a primer set and identified species-specific marker nucleotides enough to distinguish authentic Lepidii seu Descurainiae Semen and adulterants at the species level based on the matK sequences. These genetic tool will be useful to prevent adulteration and to standardize the quality of Lepidii seu Descurainiae Semen.
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KEYWORD
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Lepidii seu Descurainiae Semen, Descurainia sophia (L.) Webb ex Prantl, Lepidium apetalum Willd., Maturase K (matK), Molecular identification
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